Home Protocols Suggested Loading Protocol for N3014 & N3012

Suggested Loading Protocol for N3014 & N3012


Dilute only 2 µl of DNA Ladder at a time

If heating up the samples:

  1. Prepare loading mixture: 
    2. TE Buffer   13 μl
    Gel Loading Dye, Purple (6X), no SDS  3 μl
    DNA Ladder   2 μl
    Total volume 18 μl
  2. Mix gently by pipetting
  3. Heat samples to 60°C for 3 minutes
  4. Remove to ice or load immediately onto the agarose gel

For direct load without heating:

  1. Prepare loading mixture: 
    2. Distilled water (dH20)* or TE Buffer** 3 μl
    Gel Loading Dye, Purple (6X), no SDS 1 μl
    DNA Ladder 2 μl
    Total volume 6 μl
  2. Mix gently by pipetting
  3. Load onto the agarose gel

*For multiple loads, dilution, and storage, use TE or other buffer of minimal ionic strength instead of water. DNA may denature if diluted and stored in dH20.

**Recommended TE buffer composition: 10mM Tris-HCl, 1mM EDTA, pH8.0.

This protocol is recommended for a 5mm wide gel lane. The components of the mixture should be scaled up or down, depending on the width of the lane.