Wash Off Unbound DNA (E2600)

Protocol

1. After incubating the DNA and MBD2a-Fc/ Protein A Magnetic Beads, place the tube on the magnetic rack for 2–5 minutes to concentrate the beads on the inner wall of the tube.
2. Carefully remove the supernatant with a pipette without disturbing the beads. Save supernatant in a clean microcentrifuge tube. This saved supernatant is the non-captured DNA fraction. Store this sample on ice or at -20°C.
3. Add 1000 μl of 1X Bind/Wash Buffer to the tube to remove the residual non-captured DNA.
4. Mix the beads on a rotating mixer for 3 minutes at room temperature.
5. Place the tube on the magnetic rack for 2–5 minutes to concentrate the beads on the inner wall of the tube. Remove and discard the supernatant.
6. Repeat steps 3–5 two more times.