MBD-Fc and MCIp were originally developed by Michael Rehli at the University of Regensburg to improve the sensitivity and specificity of conventional CpG binding techniques.
Kit Components:
Each kit contains sufficient reagents for the enrichment of methylated DNA from up to 100 μg of fragmented input DNA. If starting with 5 ng to 10 μg of input DNA per experiment, the kit provides sufficient reagents for 25 reactions. Store at 4°C. For long-term storage > 6 months, MBD2-Fc protein should be stored at -20°C.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E2600S | Multi-temperature |
Bind/Wash Reaction Buffer (5X) | B1032AVIAL | 4 | 2 x 25 ml | 5 X | |
NaCl High-Salt Elution Buffer (2M) | B1033AVIAL | 4 | 1 x 15 ml | ||
Fragmented HeLa DNA | N0484AVIAL | 4 | 1 x 0.02 ml | 100 µg/ml | |
LINE Primers for Methylated Controls | N0485AVIAL | 4 | 1 x 0.02 ml | 100 µM | |
RPL30 Primers for Non-methylated Controls | N0486AVIAL | 4 | 1 x 0.02 ml | 100 µM | |
MirA Primers for Input Control | N0487AVIAL | 4 | 1 x 0.02 ml | 100 µM | |
MBD2a-Fc Protein | M0358AVIAL | -20 | 1 x 0.025 ml | 2 mg/ml | |
Protein A Magnetic Beads | S1426AVIAL | 4 | 1 x 0.25 ml |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.MBD-Fc and MCIp was developed by Michael Rehli at the University of Regensburg to improve the sensitivity and specificity of conventional CpG binding techniques.
Limited Use Label License: This product and its use is the subject of one or more issued and/or pending U.S. and foreign patent applications owned by SEQUENOM, Inc. The purchase of this product from NEB or its authorized distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any method claims in the foregoing patents or patent applications directed to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use this product for the following COMMERCIAL PURPOSES: (1) use of the product or its components in manufacturing; (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes in humans or animals; or (3) resale of the product or its components, whether or not such product or its components are resold for use in research.
ACCEPTANCE OF THE FOREGOING LIMITED USE PROVISIONS: If the purchaser is not willing to accept the limitations of this limited use statement, NEB is willing to accept return of the product with a full refund. For information on purchasing a license to use this product for purposes other than those permitted above, contact the NEB Licensing Department at busdev@neb.com.
The MBD-Fc technology is licensed exclusively to New England Biolabs from Sequenom, Inc.