The fluorescent dye can be detected using the SYBR^®/FAM channel of common real-time PCR instruments. The intercalating fluorescent dye is provided at a 50X concentration and we suggest using 0.5 μl of dye per 25 μl LAMP reaction for monitoring amplification on most real-time qPCR instruments. In some situations, further dilution of the dye may be necessary (to a final concentration of 0.1-0.5X).