Restriction Endonucleases

The Leader in the Discovery and Production of
Restriction Enzymes

After > 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme. Currently, > 250 of the restriction enzymes supplied by New England Biolabs (NEB) are recombinant proteins.

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What are Restriction Enzymes?

What are Restriction Enzymes? — Watch as Geoff Wilson, Emeritus Scientist, describes what restriction enzymes are and how they revolutionized molecular biology.

What are Enzymes for Innovation?

What are Enzymes for Innovation? — NEB’s Chief Scientific Officer, Sir Richard Roberts, describes the Enzymes for Innovation initiative, and the importance of new enzyme discovery and screening.

Discovering New Restriction Enzymes

Discovering New Restriction Enzymes — Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

Standard Protocol for Restriction Enzyme Digests

Standard Protocol for Restriction Enzyme Digests — Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.

Web Tools

Use this tool as a guide to the ever-changing landscape of restriction enzymes.

Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang.

NEBCloner web tool for Restriction Enzyme Single/Double Digestions:

Use this tool to find the right products and protocols for each step: restriction enzyme digestion, end modification, ligation and transformation.

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BSA-free Reagents

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free! Learn more about BSA-free.

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New England Biolabs – putting science first

Created "by scientists for scientists", NEB is renowned for consistently providing exceptional product quality and unsurpassed technical support.

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