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Monarch® Kits for DNA Cleanup and Gel Extraction

Monarch kits are an excellent choice for cleaning up and concentrating DNA after enzymatic reactions and from agarose gels. Highly-pure DNA is ready in minutes in volumes as low as 6 µl. Monarch kits are designed with sustainability in mind and buffers and columns are available separately for added convenience.

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Reasons to choose Monarch Kits for DNA Cleanup
Unique Column Design

Not only are Monarch columns designed to use less plastic, but they are also optimized to eliminate buffer retention; Monarch columns are designed without a retaining ring, thus preventing salts from carrying over into your eluted samples. The tapered design of the column also enables low volume elution (≥ 6 µl).

Monarch DNA Cleanup Column Design

Monarch columns are designed for performance

Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Efficient DNA Purification from Gels and Enzymatic Reactions

Monarch kits enable efficient purification of highly-pure and highly concentrated DNA from enzymatic reactions and agarose gels. Purified DNA is ready for use in downstream manipulations such as ligations and restriction digests.

Together with our enhanced buffer system, Monarch columns deliver excellent performance. Select a thumbnail to view the data for each product.

Monarch DNA Gel Extraction Kit

Gallery thumbnail Recovery, Purity & concentration Gallery thumbnail Recovery from wide range of molecular weights

Monarch PCR & DNA Cleanup Kit (5 μg)

Gallery thumbnail Recovery, Purity & concentration Gallery thumbnail Recovery, Purity & concentration
Purify oligos and small DNA fragments

The Monarch PCR & DNA Cleanup Kit protocol can be modified to enable the purification of ssDNA, oligonucleotides, and other small DNA fragments. The Oligonucleotide Cleanup protocol efficiently removes unincorporated nucleotides, short oligos, dyes, enzymes, and salts from labeling and other enzymatic reactions. The modified protocol utilizes the same columns and bind/wash/elute workflow of the Monarch PCR & DNA Cleanup Kit with > 70% recovery and cleanup of oligonucleotides ≥ 15 bp (dsDNA) or ≥18 nt (ssDNA).

Recovery of ssDNA and dsDNA oligonucleotides (1 µg) using the Oligonucleotide Cleanup Protocol and the Monarch PCR & DNA Cleanup Kit.

Synthesized ssDNA and dsDNA oligonucleotides (1 µg in 50 µl H2O) of varying lengths (6-20 nt) were purified using the Oligonucleotide Cleanup Protocol and the Monarch PCR & DNA Cleanup Kit (NEB #T1030) and were eluted in 50 µl water. The average percent recovery (n=3) of the oligonucleotides was calculated from the resulting A260 as measured using a Trinean DropSense™ 16. Use of the Oligonucleotide Cleanup Protocol and the Monarch PCR & DNA Cleanup Kit (NEB #T1030) results in the efficient removal of small oligonucleotides (6-12 nt) and > 70% recovery and cleanup of oligonucleotides ≥ 15 bp (dsDNA) or ≥ 18 nt (ssDNA).
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
Monarch® DNA Gel Extraction Kit protocol
DNA Gel Extraction Kit protocol
Learn how to extract DNA from agarose gels using the Monarch DNA Gel Extraction Kit.
Monarch® PCR & DNA Cleanup Kit Protocol
PCR & DNA Cleanup Kit Protocol
Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).
Tips for using the Monarch® DNA Gel Extraction Kit
DNA Gel Extraction Kit
Optimize your DNA gel extractions with our quick tips for using the Monarch DNA Gel Extraction Kit.
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I often purify small amounts of inserts for ligations, so I appreciate the ability to elute in small volumes. I also very much appreciate the reduction of paper and plastic waste.

– Research Associate, Indiana University

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