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HeLa Genomic DNA

Catalog # Concentration Size List Price Quantity Your Price
N4006S 100 µg/ml 15 µg
Please Inquire
Catalog # Size List Price Your Price
N4006S 15 µg
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Highly pure HeLa Genomic DNA for use in a wide variety of applications including: PCR, SNP analysis and southern blotting
  • Genomic DNA library construction
  • Control DNA for Methylation-specific PCR (MSP), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (MS-SNuPE,) Combined Bisulfite Restriction Analysis (COBRA), Bisulfite treatment and PCR-single strand Confirmation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS)
Human female HeLa genomic DNA.

Product Source:
 
HeLa (cervix adenocarcinoma) cells were grown to confluency in DMEM plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol (1), phenol extracted and equilibrated to 10 mM TrisHCl (pH 7.5) and 1 mM EDTA.
Application Features
  • A control for Methylation-Specific PCR (MSP) (2), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE), Combined Bisulfite Restriciton Analysis (COBRA), Bisulfite treatment and PCR-Single-Strand Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS).
  • PCR, SNP Analysis, Southern Blotting
  • Genomic DNA library construction

Properties & Usage

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C



Companion Products
Notes
  • Avoid multiple freeze/thaw cycles.
References
  • Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), (pp. 6.4-6.12). Cold Spring Harbor: Cold Spring Ha.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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