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EnGen® Spy dCas9 SNAP tag

Catalog # Concentration Size List Price Quantity Your Price
M0652S 1 µM 90 pmol $91.00
$81.90
M0652T 20 µM 500 pmol $229.00
$206.10
Catalog # Size List Price Your Price
M0652S 90 pmol $91.00
$81.90
M0652T 500 pmol $229.00
$206.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • An inactive mutant of Cas9 nuclease that retains programmable DNA binding activity
  • The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment
EnGen® Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity (1,2). EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag® allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
Product Source
EnGen® Spy dCas9 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  EnGen® Spy dCas9 (SNAP-tag®) M0652SVIAL -20 1 x 90 pmol 1 µM
  NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X
  EnGen® Spy dCas9 (SNAP-tag®) M0652TVIAL -20 1 x 500 pmol 20 µM
  NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage

Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Storage Buffer

300 mM NaCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.4 @ 25°C



Companion Products
Notes
  • 20 µM is equal to ~3.6 mg/ml and 1uM is equal to ~ 0.18 mg/ml EnGen® Spy dCas9.
  • This product is compatible with Diluent Buffer B: 300 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 500 µg/ml BSA and 50% glycerol. (pH 7.4 @ 25°C).
References
  • Qi, LS., et. al. (2013). Cell. 152 (5), 1173-83.
  • Qi, LS., et. al. (2013). Cell. 154 (2), 442-51.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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