For most applications, especially where time-tunable fragmentation or preservation of methylation is needed, we recommend NEBNext UltraShear®.
Note: NEBNext dsDNA Fragmentase now has an updated protocol and components list.
NEBNext dsDNA Fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols
Note: NEBNext dsDNA Fragmentase is not part of the NEBNext Ultra™ II FS (Fragmentation System) reagents.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.
Reaction Definition: One reaction is defined as the amount of NEBNext dsDNA Fragmentase required to convert 1 µg of purified HeLa cell gDNA in 20 µl of 1X NEBNext dsDNA Fragmentase Reaction Buffer v2 into short (100–300 bp) DNA fragments in 30 minutes at 37°C.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
1X NEBNext® dsDNA Fragmentase® Reaction Buffer v2
1X NEBNext® dsDNA Fragmentase® Reaction Buffer v2
20 mM Tris-HCl
15 mM MgCl2
50 mM NaCl
0.1 mg/ml BSA
0.15% Triton® X-100
(pH 7.5 @ 25°C)
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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