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NEBNext® dsDNA Fragmentase®

Catalog # Concentration Size List Price Quantity Your Price
M0348L 250 reactions $677.00
$609.30
M0348S 50 reactions $170.00
$153.00
Catalog # Size List Price Your Price
M0348L 250 reactions $677.00
$609.30
M0348S 50 reactions $170.00
$153.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext dsDNA Fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols

  • dsDNA Fragmentase provides random fragmentation, similar to mechanical methods (1,2)

Note: NEBNext dsDNA Fragmentase is not part of the NEBNext Ultra™ II FS (Fragmentation System) reagents.





NEBNext® dsDNA Fragmentase generates dsDNA breaks in a time-dependent manner to yield 50–1,000 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, thereby producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl groups. The random nicking activity of NEBNext dsDNA Fragmentase has been confirmed by preparation of libraries for next-generation sequencing. A comparison of the sequencing results between libraries prepared with genomic DNA sheared with NEBNext dsDNA Fragmentase and with mechanical shearing demonstrates that NEBNext dsDNA Fragmentase does not introduce any detectable bias during sequencing library preparation and no difference in sequence coverage is observed between the two methods.


For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

Reaction Definition:  One reaction is defined as the amount of NEBNext dsDNA Fragmentase required to convert 1 µg of purified HeLa cell gDNA in 20 µl of 1X NEBNext dsDNA Fragmentase Reaction Buffer v2 into short (100–300 bp) DNA fragments in 30 minutes at 37°C.

 


Figure 1: Fragmentation of E. coli gDNA
Figure 1: Fragmentation of E. coli gDNA.
E. coli gDNA was fragmented with NEBNext dsDNA Fragmentase for the indicated times and purified on MinElute® columns.
Figure 2: Fragmentation
Figure 2: Fragmentation
Fragmentation of 5,10 and 20 kb amplicons with NEBNext dsDNA Fragmentase.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® dsDNA Fragmentase® M0348AVIAL -20 1 x 50 reactions
  NEBNext® dsDNA Fragmentase® Reaction Buffer v2 B0349AVIAL -20 1 x 0.1 ml 10 X
  200mM MgCl2 B0511AVIAL -20 1 x 0.1 ml
  NEBNext® dsDNA Fragmentase® M0348AAVIAL -20 1 x 250 reactions
  NEBNext® dsDNA Fragmentase® Reaction Buffer v2 B0349AAVIAL -20 1 x 0.5 ml 10 X
  200mM MgCl2 B0511AAVIAL -20 1 x 0.5 ml
Application Features
  • Generation of dsDNA fragments for sequencing on next generation sequencing platforms
  • Generation of dsDNA fragments for libraries

Properties & Usage

Reaction Conditions

1X NEBNext® dsDNA Fragmentase® Reaction Buffer v2

1X NEBNext® dsDNA Fragmentase® Reaction Buffer v2
20 mM Tris-HCl
15 mM MgCl2
50 mM NaCl
0.1 mg/ml BSA
0.15% Triton® X-100
(pH 7.5 @ 25°C)



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The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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