The NEBNext dA-Tailing Module enables incorporation of a non-templated dAMP on the 3´ end of a blunt-ended DNA fragment. The module is optimized for use with the NEBNext Quick Ligation Module (NEB #E6056), and is part of the original standard DNA library prep workflow for Illumina sequencing, which is suitable for 1–5 µg of input DNA.
Each kit component must pass rigorous quality control standards, and for each new lot the entire set of reagents is functionally validated together by construction and sequencing of an indexed library on the Illumina sequencing platform.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
Thermocycler
AMPure® XP Beads (Beckman Coulter, Inc. #A63881) or SPRIselect® Reagent Kit (Beckman Coulter, Inc. #B23317)
10 µM Tris-HCl, pH 7.5-8.0 or 0.1 µM Tris-HCl, pH 8.0
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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