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NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1)

Catalog # Concentration Size List Price Quantity Your Price
E9725L 96 reactions $780.00
$702.00
E9725S 24 reactions $194.00
$174.60
Catalog # Size List Price Your Price
E9725L 96 reactions $780.00
$702.00
E9725S 24 reactions $194.00
$174.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) enables high-yield, multiplexed MGI library production. The carefully selected pairs of index primers (following a combinatorial dual indexing scheme) are provided pre-mixed in a 96-well plate for ease of use. The small pack (NEB #E9725S) contains 24 pairs of index primers, while the large pack (NEB #E9725L) contains 96 pairs of index primers; the S and L packs may be combined for a total of 120 index primer pairs.

  • Convenient pre-selected pairs of primers for multiplex library prep for MGI
  • Can be combined for indexing of up to 120 samples
  • Single-use, pre-mixed wells ensure maximal purity with minimal cross contamination
The primer pairs in NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) are used to incorporate index sequences (sample barcodes) into library molecules during PCR. The addition of these sample indices enables multiplexing; Set 1 allows for the multiplexing of up to 120 samples.

For sample sheets for use with MGI sequencers, scroll down to Usage Guidelines below "Protocols, Manuals & Usage".
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Dual Index Primer Pairs for MGI® E9648AVIAL -20 1 x 6 μl/well 20 µM
  NEBNext® Adaptor for MGI® E9650AVIAL -20 1 x 0.12 ml 40 µM
  NEBNext® Adaptor Dilution Buffer for MGI® E9651AVIAL -20 1 x 0.25 ml
  NEBNext® Dual Index Primer Pairs for MGI® E9649AVIAL -20 1 x 6 μl/well 20 µM
  NEBNext® Adaptor for MGI® E9650AAVIAL -20 1 x 0.48 ml 40 µM
  NEBNext® Adaptor Dilution Buffer for MGI® E9651AAVIAL -20 1 x 1 ml
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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