NEBNext® FS DNA Library Prep Kit for MGI®

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E9705L		96 reactions	$2,645.00		$2,380.50
E9705S		24 reactions	$734.00		$660.60

Catalog #	Size	List Price	Your Price
E9705L	96 reactions	$2,645.00	$2,380.50
E9705S	24 reactions	$734.00	$660.60

The NEBNext FS DNA Library Prep Kit for MGI meets the need for reliably high-quality DNA library prep from intact samples for MGI sequencing applications.

The kit incorporates trusted NEBNext master mixed reagents and protocols, allowing the use of a wide range of intact DNA inputs (0.1 - 500 ng). The integrated enzymatic fragmentation, end-repair, and dA-tailing step offers fragment-size tunability with a single enzyme mix; the "FS" stands for Fragmentation System.

Note that adaptors, primers, and circularization reagents are provided separately in the NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) (NEB #E9725) and NEBNext Circularization Module for MGI (NEB #E9720).

Get more of what you need with high library yields
Generate high-quality libraries with flexible input amounts from 0.1 ng to 500 ng of intact DNA
Appreciate the convenience of a single adaptor-dilution recommendation
Optimize your time with streamlined workflows, minimized hands-on time, and automation compatibility
Rely on robust performance

Workflow for E9705 — *The NEBNext FS DNA Library Prep Kit for MGI has a streamlined workflow with minimal hands-on time across a range of inputs from 0.1 ng to 500 ng.*

E9705 Yield — Libraries were prepared from 0.1–500 ng of human gDNA (NA12878, Coriell Institute for Medical Research) with the indicated PCR cycles. Size selection was performed for a 250 bp average fragment size, except for the 0.1 ng input, where bead cleanup was conducted to avoid excess sample loss and to preserve library complexity. Stock adaptor concentration was used for all input amounts except for 0.1 ng, where a 10-fold dilution of the stock was applied. No traces of adaptor dimer were observed (at ~150 bp) at all input levels (A). Robust library yields were obtained across input amounts. Each bar represents the average of 8–18 replicates, with error bars indicating standard deviation (B). M = Marker

E7905 Prep — Libraries were prepared in duplicate from 0.1–500 ng of NA12878 gDNA (Coriell Institute for Medical Research), sequenced on an MGI^® G99 platform with PE100 and downsampled to 3.5 million paired-end reads. Reads were mapped using Bowtie2 (version 2.3.2.2) to the GRCh38 reference, and duplicates were marked using Picard MarkDuplicates (version 1.56.0). Library quality metrics were assessed using Picard Alignment Summary Metrics (version 1.56.0). High mapping rates, low chimera rates, and low adaptor-dimer rates were obtained for all libraries. A slightly higher adaptor-dimer presence (~1%) was observed with the 1 ng input for this specific experiment, and this can be optimized by adaptor dilution. Stock adaptor concentration was used for all input levels except for 0.1 ng, where a 10-fold dilution of the stock was applied.

E7905 Coverage — Libraries were prepared in duplicate from 0.1–500 ng of NA12878 gDNA (Coriell Institute for Medical Research), sequenced on an MGI^® G99 platform with PE100 and downsampled to 3.5 million paired-end reads. Reads were mapped using Bowtie2 (version 2.3.2.2) to the GRCh38 reference, and duplicates were marked using Picard MarkDuplicates (version 1.56.0). GC coverage was calculated using Picard's CollectGCBiasMetrics (1.56), which was run on human autosomes only, due to the even copy-number assumption of the tool. Uniform GC coverage was achieved for all input amounts. In the GC coverage plot, the horizontal grey lines indicate the expected normalized coverage of 1.0, and the dots in shades of green represent read numbers at each GC%. The grey area plots below are histograms representing the distribution of GC content in 100 bp windows of the reference genome.

E7905 Inputs — Libraries were prepared in duplicate from 0.1–500 ng of NA12878 gDNA (Coriell Institute for Medical Research) with a 250 bp size selection performed for 1–500 ng, or a simple bead cleanup step for 0.1 ng to preserve library complexity. Libraries were pooled and sequenced on an MGI G99 platform with 2x100 base reads and downsampled to 3.5 million paired-end reads. Reads were mapped using Bowtie2 (version 2.3.2.2) to the GRCh38 reference, and insert size distributions were calculated using Picard CollectInsertSizeMetrics (v1.56.0). Consistent insert sizes of 250 bp were achieved for all input amounts with size selection. In contrast, a smaller size distribution was observed for inputs of 0.1 ng where a bead-based clean-up step was used to remove adaptor dimers and excess adaptors, without size selection.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E9705S -20

NEBNext^® FS Reaction Buffer	E9616AVIAL	-20	1 x 0.168 ml
NEBNext^® FS Enzyme Mix	E9617AVIAL	-20	1 x 0.048 ml
NEBNext^® Ligation Master Mix	E9613AVIAL	-20	1 x 0.72 ml
NEBNext^® Ligation Enhancer	E7374AVIAL	-20	1 x 0.024 ml
NEBNext^® MSTC™ High Yield Master Mix	E9615AVIAL	-20	1 x 0.6 ml
TE Buffer (1X)	E9614AVIAL	-20	1 x 1.5 ml

E9705L -20

NEBNext^® FS Reaction Buffer	E9616AAVIAL	-20	1 x 0.672 ml
NEBNext^® FS Enzyme Mix	E9617AAVIAL	-20	1 x 0.192 ml
NEBNext^® Ligation Master Mix	E9613AAVIAL	-20	3 x 0.96 ml
NEBNext^® Ligation Enhancer	E7374AAVIAL	-20	1 x 0.096 ml
NEBNext^® MSTC™ High Yield Master Mix	E9615AAVIAL	-20	2 x 1.2 ml
TE Buffer (1X)	E9614AAVIAL	-20	1 x 6 ml

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products

Protocols

Where can I find protocols for the NEBNext® FS DNA Library Prep kit for MGI® (NEB #E9705)?

DataCards

manualE9705

FAQs

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

NEBNext^® Ligation Enhancer

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.