NEBNext® Enzymatic Methyl-seq v2 Conversion Module

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E8020L		96 reactions	$996.00		$896.40
E8020S		24 reactions	$273.00		$245.70

Catalog #	Size	List Price	Your Price
E8020L	96 reactions	$996.00	$896.40
E8020S	24 reactions	$273.00	$245.70

NEBNext Enzymatic Methyl-seq (EM-seq™) is a high-performance enzyme-based alternative to bisulfite conversion for the identification of 5mC and 5hmC. Unlike bisulfite conversion, this highly efficient method minimizes DNA damage, resulting in superior detection of methylated cytosines, with fewer sequencing reads.

The new NEBNext Enzymatic Methyl-seq v2 Conversion Module has a wider input range (as low as 100 pg) than the original EM-seq Conversion Module (NEB #E7125).

For use in Illumina^® sequencing workflows, we recommend use of the NEBNext Enzymatic Methyl-seq v2 Kit (NEB #E8015) instead of this module. The full kit includes the EM-seq Adaptor, which has been optimized for high performance in the EM-seq workflow, as well as the high-efficiency NEBNext Ultra™ II library prep reagents, and these components are not included in the Conversion Module.

High efficiency conversion
0.1 ng - 200 ng input range
Minimal DNA damage
Minimized GC bias
5mC and 5hmC detection
Complete kit including library prep reagents also available

For specific detection of 5hmC, the NEBNext Enzymatic E5hmC-seq Conversion Module (NEB #E3365) is also available.

View or download extensive EM-seq v2 performance data in our Data Supplement.

The NEBNext Enzymatic Methyl-seq v2 Conversion Module provides a high-performance enzyme-based alternative to bisulfite conversion for methylome analysis, with as little as 100 pg of input DNA.
EM-seq is a two-step enzymatic conversion process to detect modified cytosines. In the first step, TET2 and T4-BGT protect modified cytosines from downstream deamination. TET2 enzymatically oxidizes 5mC and 5hmC, and T4-BGT glucosylates 5hmC.

The second enzymatic step uses APOBEC to deaminate unmodified cytosines to uracils, but 5mC and 5hmC protected in the first step are not deaminated.

For specific detection of 5hmC, the NEBNext Enzymatic E5hmC-seq Conversion Module (NEB #E3365) is also available.

Diagram showing sodium bisulfite conversion method versus EM-seq conversion method — *The EM-seq™ v2 workflow accommodates a wider input range than the original EM-seq workflow, with a 100-fold lower minimum input amount.*

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E8020S -20

Control DNA CpG methylated pUC19	E7122AVIAL	-20	1 x 0.024 ml	0.1 ng/µl
Control DNA CpG unmethylated Lambda	E7123AVIAL	-20	1 x 0.024 ml	2 ng/µl
NEBNext^® Carrier DNA	E3351AVIAL	-20	1 x 0.024 ml
Elution Buffer	E7124AVIAL	-20	1 x 2.1 ml
TET2 Reaction Buffer	E7126AVIAL	-20	1 x 0.3 ml
TET2 Reaction Buffer Supplement (x 1)	E8013AVIAL	-20	3 x 100 µl
UDP-Glucose	E3353AVIAL	-20	1 x 0.024 ml
DTT	E7139AAVIAL	-20	1 x 0.5 ml	100 mM
T4-BGT	E3354AVIAL	-20	1 x 0.024 ml
T4-BGT Diluent	E8014AVIAL	-20	1 x 0.216 ml
TET2	E7130AVIAL	-20	1 x 0.096 ml
Fe (II) Solution	E7131AVIAL	-20	1 x 0.024 ml
Stop Reagent	E7132AVIAL	-20	1 x 0.024 ml
APOBEC	E7133AVIAL	-20	1 x 0.024 ml
Deamination Reaction Buffer	E3356AVIAL	-20	1 x 0.096 ml
Recombinant Albumin	E3357AVIAL	-20	1 x 0.024 ml

E8020L -20

Control DNA CpG methylated pUC19	E7122AAVIAL	-20	1 x 0.096 ml	0.1 ng/µl
Control DNA CpG unmethylated Lambda	E7123AAVIAL	-20	1 x 0.096 ml	2 ng/µl
NEBNext^® Carrier DNA	E3351AAVIAL	-20	1 x 0.096 ml
Elution Buffer	E7124AAVIAL	-20	1 x 8.6 ml
TET2 Reaction Buffer	E7126AAVIAL	-20	1 x 1.2 ml
TET2 Reaction Buffer Supplement (x 1)	E8013AAVIAL	-20	3 x 400 µl
UDP-Glucose	E3353AAVIAL	-20	1 x 0.096 ml
DTT	E7139AAVIAL	-20	1 x 0.5 ml	100 mM
T4-BGT	E3354AAVIAL	-20	1 x 0.096 ml
T4-BGT Diluent	E8014AAVIAL	-20	1 x 0.864 ml
TET2	E7130AAVIAL	-20	1 x 0.384 ml
Fe (II) Solution	E7131AAVIAL	-20	1 x 0.096 ml
Stop Reagent	E7132AAVIAL	-20	1 x 0.096 ml
APOBEC	E7133AAVIAL	-20	1 x 0.096 ml
Deamination Reaction Buffer	E3356AAVIAL	-20	1 x 0.384 ml
Recombinant Albumin	E3357AAVIAL	-20	1 x 0.096 ml

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products

FAQs

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

E8020S_L_G_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.