NEBNext LV Unique Dual Index Primers Set 5 includes 96 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ v2 and E5hmC-seq™, with Illumina® sequencing. The primers enable incorporation of unique index pairs during library amplification and have been designed to minimize index hopping.
NEBNext LV Unique Dual Index Primers have been selected and paired for optimal multiplex index incorporation during PCR. Optimized for low-volume (LV) NEBNext workflows, including EM-seq v2 and E5hmC-seq, these primers enable multiplexing and have been designed to minimize index hopping. They are supplied as pairs of premixed i5 and i7 8-base index primers in a 96-well plate that has a pierceable foil seal for easy, single use.
The index sequences from NEBNext LV Unique Dual Index Primers Set 4 are compatible with and can be combined with the other NEBNext LV Unique Dual Index Primers sets to multiplex up to 480 reactions. Please refer to the relevant NEBNext library prep kit manual for usage instructions. Sample sheets for use with these NEBNext LV Unique Dual Index Primers can be found under "Usage Guidelines" on this product page (see below).
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