The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.
NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520) or the Gibson Assembly Cloning Kit (NEB #E5510) are excellent ways to assemble a linear yeast expression cassette. (See "Improved method for assembly of linear yeast expression cassettes using NEBuilder® HiFi DNA Assembly Master Mix" in the Application Notes section under the Other Tools and Resources tab)
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Notice to Buyer/User: The K. lactis Protein Expression Kit was developed from basic research at New England Biolabs, Inc. and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.