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Yeast Carbon Base Medium Powder

Catalog # Concentration Size List Price Quantity Your Price
B9017S 12 g $77.00
$69.30
Catalog # Size List Price Your Price
B9017S 12 g $77.00
$69.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
This medium pack includes 12 grams of Yeast Carbon Base Medium Powder and 10 ml of Acetamide Solution.

The Yeast Carbon Base Medium Powder contains sufficient reagents needed to make 1 liter of Yeast Carbon Base (YCB) Agar Medium containing 5 mM acetamide. 

YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Yeast Carbon Base Medium Powder (12 g) B9011SVIAL 4 1 x 12 g
  Acetamide solution (sterile) (10 ml) B9018SVIAL 4 1 x 10 ml 100 X
Yeast Carbon Base Medium Powder | New England Biolabs
Home Buffers Products Yeast Carbon Base Medium Powder

Yeast Carbon Base Medium Powder

This medium pack includes 12 grams of Yeast Carbon Base Medium Powder and 10 ml of Acetamide Solution.

The Yeast Carbon Base Medium Powder contains sufficient reagents needed to make 1 liter of Yeast Carbon Base (YCB) Agar Medium containing 5 mM acetamide. 

YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB https://www.neb.com/en/#N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
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Catalog https://www.neb.com/en/# Concentration Size
B9017S Not Applicable 12 g
Please enter a quantity for at least one size

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Tech Tips
  • Make sure agar is cooled to ~50 C before adding acetamide
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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