Source
Quick-Load Purple 1 kb Plus DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
| Fragment | Mass (ng) | bp |
|---|---|---|
| 1 | 40 | 10,002 |
| 2 | 40 | 8,001 |
| 3 | 48 | 6,001 |
| 4 | 40 | 5,001 |
| 5 | 32 | 4,001 |
| 6 | 120 | 3,001 |
| 7 | 40 | 2,017 |
| 8 | 57 | 1,517 |
| 9 | 45 | 1,200 |
| 10 | 122 | 1,000 |
| 11 | 34 | 900 |
| 12 | 31 | 800 |
| 13 | 27 | 700 |
| 14 | 23 | 600 |
| 15 | 124 | 500/517 |
| 16 | 49 | 400 |
| 17 | 37 | 300 |
| 18 | 32 | 200 |
| 19 | 61 | 100 |
2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8 @ 25°C
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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