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Monarch® Spin RNA Cleanup Kit (10 μg)

Catalog # Concentration Size List Price Quantity Your Price
T2030L 100 preps $468.00
$421.20
T2030S 10 preps $92.00
$82.80
Catalog # Size List Price Your Price
T2030L 100 preps $468.00
$421.20
T2030S 10 preps $92.00
$82.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The Monarch Spin RNA Cleanup Kit (10 µg) enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
  • Elute in ≥ 6 µl for concentrated RNA
  • 70-100% RNA recovery, even with inputs < 20 ng
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
  • Simplified workflow with a single wash buffer
  • Unique column design helps prevent buffer carryover and elution of silica particulates
  • Purified RNA is ready for use in a wide variety of downstream applications

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Monarch® RNA Cleanup Protocol
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for successful RNA Cleanup using the Monarch® RNA Cleanup Kits
  • Monarch_MiniprepProtocol_VideoThumb
    Avoiding RNase Contamination
  • Monarch_MiniprepProtocol_VideoThumb
    How to recycle your Monarch® Kit components

The Monarch Spin RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch Spin RNA Cleanup Kits are also available for 50 µg (NEB #T2040) and 500 µg (NEB #T2050) binding capacities. Columns are also available separately for convenience.

 

Figure 1: Monarch Spin RNA Cleanup Kit workflow

 

Specifications and Applications:

SPECIFICATIONS
RNA Sample Type Cleanup and concentration of RNA from enzymatic reactions (labeling, capping, in vitro transcription reactions, DNase I treatment)
Binding Capacity 10 μg
RNA Size Range ≥ 25 nt ( ≥ 15 nt with modified protocol)
Typical Recovery 70–100%
Elution Volume 6–20 µl
Purity A260/280 > 1.8 and A260/230 > 1.8
Protocol Time 5 minutes of spin and incubation time
Compatible Downstream Applications RT-PCR, Small RNA library prep for NGS, RNA Library Prep for NGS

APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase) RNA purified by other methods can be further purified
Enzymatic Reaction Cleanup Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
In vitro Transcription Cleanup Enzymes and excess NTPs are removed to yield highly pure synthesized RNA
RNA Gel Extraction Purification of RNA from agarose gels
RNA Fractionation Fractionation of RNA into small and large RNA pools

 

Figure 2: The Monarch Spin RNA Cleanup Kit enables efficient recovery of RNA in as little as 6 µl

rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch Spin RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl.
Figure 3: The Monarch Spin RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl

A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch Spin RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.  
Figure 4: The Monarch Spin RNA Cleanup Kit (10 µg) offers efficient recovery of sample volume

 

The Monarch Spin Columns for RNA Cleanup Kit (10 µg, NEB #T2037) ensure zero buffer retention and no carryover of contaminants, enabling sample elution in volumes as low as 6 µl. Moreover, ~90% (~5.4 µl) of the minimal elution volume is recovered from these Monarch columns, compared to other competitor kits.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Monarch Spin Columns S1A T2037-21 25 1 x 10 columns
  Monarch® Spin Collection Tubes T2118-21 25 1 x 10 tubes
  Monarch® Buffer BX T2041-21 25 1 x 3 ml
  Monarch® Buffer WX T2042-21 25 1 x 2.5 ml
  Nuclease-free Water B1500-21 25 1 x 2.5 ml
  Monarch Spin Columns S1A T2037-1 25 2 x 50 columns
  Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes
  Monarch® Buffer BX T2041-2 25 1 x 40 ml
  Monarch® Buffer WX T2042-2 25 2 x 20 ml
  Nuclease-free Water B1500-2 25 1 x 25 ml

Properties & Usage



FAQs
Troubleshooting
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications

Effective September 24, 2024, the product name modified to Monarch® Spin RNA Cleanup Kit (10 μg). The component/product name of Monarch RNA Cleanup Columns has changed to Monarch Spin Columns S1A.

Effective March 25, 2024, component/product name changed to Monarch® Buffer BX. Product specifications have been updated.

Effective March 25, 2024, component/product name changed to Monarch® Buffer WX. Product specifications have been updated.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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