RNase B

Catalog # Concentration Size List Price Quantity Your Price
P7817S 5 mg/ml 250 µg $115.00
$103.50
Catalog # Size List Price Your Price
P7817S 250 µg $115.00
$103.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

RNase B is a high mannose glycoprotein that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates.

  •  Has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays
  • Optimal stability for up to 24 months


 
RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.

Product Source
Bovine pancreas
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  RNase B P7817SVIAL -20 1 x 0.05 ml 5 mg/ml

Properties & Usage

Storage Buffer

50 µl Milli-Q water
pH 7 @ 25°C

Molecular Weight

Theoretical: 17000 daltons


References
  • Plummer, T.H. and Hirs, C. (1964). J. Biol. Chem. 239, 2530-2538.
  • Plummer, T.H. Jr., Tarentino, A. and Maley, F. (1968). J. Biochem. (Tokyo). 243, 5158-5164.
  • Liang, C.-J. Yamashita, K. and Kobata, A. (1980). J. Biochem (Tokyo). 88, 51-58.
Tech Tips
  • This is a good positive control for Endo H, Endo Hf, PNGase F and PNGase F Glycerol Free
    250 µg is enough for approximately 20 reactions
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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