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Endo S

Catalog # Concentration Size List Price Quantity Your Price
P0741L 200000 units/ml 30000 units $1,273.00
$1,145.70
P0741S 200000 units/ml 6000 units $320.00
$288.00
Catalog # Size List Price Your Price
P0741L 30000 units $1,273.00
$1,145.70
P0741S 6000 units $320.00
$288.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG

  • Active on native IgG
  • Tagged with a chitin binding domain (CBD) for easy removal
  • Recombinant enzyme with no detectable exooglycosidase or other endoglycosidases contaminating activities
  • Glycerol -free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
  • Optimal activity and stability for up to 12 months


 
Endo S is an endoglycosidase with a uniquely high specificity for removing N-linked glycans from the chitobiose core of the heavy chain of native IgG (1). Endo S is tagged with a chitin binding domain (CBD) for easy removal from a reaction and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.

Product Source
Endo S is cloned from Streptococcus pyogenes and overexpressed as a fusion to the chitin binding domain in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Endo S P0741SVIAL 4 1 x 0.03 ml 200,000 units/ml
  GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X
  Endo S P0741LVIAL 4 1 x 0.15 ml 200,000 units/ml
  GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.

Reaction Conditions

1X GlycoBuffer 1
Incubate at 37°C

1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)

Storage Buffer

20 mM Tris-HCl
50 mM NaCl
5 mM EDTA
pH 7.5 @ 25°C

Heat Inactivation

55°C for 10 minutes

Molecular Weight

Apparent: 136000 daltons

Unit Assay Conditions

5 µg of IgG in 1X GlycoBuffer 1 are incubated with two-fold dilutions of Endo S for 1 hour at 37°C. Separation of reaction products is visualized by SDS-PAGE.

Companion Products
Notes
  • Recommended storage temperature is 4°C,avoid repeat freeze-thaw cycles.
  • Removal of Endo S from thedeglycosylation reaction can be scaled uplinearly with larger volumes of chitin magneticbeads.
  • The binding capacity of Chitin Magnetic Beads is 1 mg of CBD-tagged protein / mL of beads.
References
  • Bielik, A., McLeod, E. and Magnelli, P., New England Biolabs,Inc., unpublished results.. Unpublished observation
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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