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T7 Endonuclease I

Catalog # Concentration Size List Price Quantity Your Price
M0302L 10000 units/ml 1250 units $467.00
$420.30
M0302S 10000 units/ml 250 units $117.00
$105.30
Catalog # Size List Price Your Price
M0302L 1250 units $467.00
$420.30
M0302S 250 units $117.00
$105.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • DNA Endonuclease
  • Catalyzes the cleavage of DNA mismatches and non-β DNA structures including Holliday junctions and cruciform leaving 3'-OH and 5'-phosphate
  • Best at C mismatches and does not recognize all DNA mismatches
  • To a lesser extent cleaves across a nick in dsDNA
  • Key enzyme for use in genome editing mutation detection workflows
  • Also available in convenient kit format: EnGen Mutation Detection Kit (NEB #E3321)
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    What are endonucleases and their applications?
  • Monarch_MiniprepProtocol_VideoThumb
    Quick Tips - Why don't my bands on a gel look correct after T7 digestion?
  • Monarch_MiniprepProtocol_VideoThumb
    Quick Tips - Should I clean up my PCR before T7 endonuclease digestion?
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3.
Highlights
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Recognizes non-perfectly matched DNA
Product Source
An E. coli strain that carries a fusion of maltose binding protein and T7 Endonuclease I (T7 Endo I).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  T7 Endonuclease I M0302SVIAL -20 1 x 0.025 ml 10,000 units/ml
  NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X
  T7 Endonuclease I M0302LVIAL -20 1 x 0.125 ml 10,000 units/ml
  NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X
Application Features
  • Resolve four-way junction or branched DNA
  • Detect or cleave heteroduplex and nicked DNA
  • Randomly cleave linear DNA for shot-gun cloning
T7 Endonuclease I | New England Biolabs

T7 Endonuclease I
NEB2 cloned at NEB recombinant 37 No

Authenticase® (NEB https://www.neb.com/en/#M0689) now available for improved genome editing mutation detection.

  • DNA Endonuclease
  • Catalyzes the cleavage of DNA mismatches and non-β DNA structures including Holliday junctions and cruciform leaving 3'-OH and 5'-phosphate
  • Best at C mismatches and does not recognize all DNA mismatches
  • To a lesser extent cleaves across a nick in dsDNA
  • Key enzyme for use in genome editing mutation detection workflows
  • Also available in convenient kit format: EnGen Mutation Detection Kit (NEB https://www.neb.com/en/#E3321)
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Featured Video

Catalog https://www.neb.com/en/# Concentration Size
M0302S 10,000 units/ml 250 units
M0302L 10,000 units/ml 1,250 units
Please enter a quantity for at least one size

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Companion Products
Materials Sold Separately
Notes
  • T7 Endonuclease I is a structure-selective enzyme. It acts on a variety of DNA substrates with different specific activities.
  • It is important to control the amount of enzyme and the reaction time used for cleavage of a particular substrate.
  • Temperatures above 42°C cause an increase in nonspecific nuclease activity and should be avoided.
  • pUC(AT) is derived from pUC19 with a modification of the polylinker between the EcoRI site and the PstI site.
References
  • Xu, M. -Q. and Evans, T.C. (2001). Methods. 24, 257-277.
  • Parkinson, M.J. and Lilley. D.M.J. (1997). J. Mol. Biol.. 270, 169-178.
  • White, M.F. et al. (1997). J. Mol. Biol.. 269, 647-664.
  • Hadden, J.M. et al. (2001). Nat. Struct. Biol.. 8, 62-67.
Citations
Additional Citations
  • Zheng Hu, Wencheng Ding, Da Zhu, Lan Yu, Xiaohui Jiang, Xiaoli Wang, Changlin Zhang, Liming Wang, Teng Ji, Dan Liu, Dan He, Xi Xia, Tao Zhu, Juncheng Wei, Peng Wu, Changyu Wang, Ling Xi, Qinglei Gao, Gang Chen, Rong Liu, Kezhen Li, Shuang Li, Shixuan Wang, Jianfeng Zhou, Ding Ma, Hui Wang (2015) TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. J Clin Invest 125, 425-36.PubMedID: 25500889, DOI: 10.1172/JCI78206
  • A-Bk Dad, S Ramakrishna, M Song, H Kim (2014) Enhanced gene disruption by programmable nucleases delivered by a minicircle vector. Gene Ther ,PubMedID: 25142139, DOI: 10.1038/gt.2014.76
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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