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m7G(5')ppp(5')G RNA Cap Structure Analog

Catalog # Concentration Size List Price Quantity Your Price
S1404L 5 µmol $1,056.00
$950.40
S1404S 1 µmol $265.00
$238.50
Catalog # Size List Price Your Price
S1404L 5 µmol $1,056.00
$950.40
S1404S 1 µmol $265.00
$238.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

 

  • The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). 
  • A method for efficient in vitro synthesis of capped RNA using E.coli RNA polymerase primed with m7G(5´ )ppp(5´ )G (#S1404S) or m7G(5´ )ppp(5´ )A (#S1405S) has been developed by Contreas et al. (7).
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    Avoiding RNase Contamination

For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

 

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  m7G(5')ppp(5')G RNA Cap Structure Analog S1404SVIAL -20 1 x 1 µmol
  m7G(5')ppp(5')G RNA Cap Structure Analog S1404SVIAL -20 5 x 1 µmol

Properties & Usage

Supplied As

Dried Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

Notes
  • Addition of 100 µl of water gives approximately a 10 mM solution.
References
  • Shatkin, A.J. (1978). Cell. 9, 645-653.
  • Fillipowicz, W. (1978). FEBS Lett.. 96, 1-11.
  • Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
  • Miura, K. (1981). Adv. Biophys.. 14, 205-238.
  • Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
  • Konarska, M.M. et al. (1984). Cell. 38, 731-736.
  • Contreas, R. et al. (1982). Nucl. Acids Res.. 10, 6353-2363.
  • Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.
Quality Control Assay
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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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