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TEV Protease

Catalog # Concentration Size List Price Quantity Your Price
P8112S 10000 units/ml 1000 units $173.00
$155.70
Catalog # Size List Price Your Price
P8112S 1000 units $173.00
$155.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The TEV protease recognition sequence with the highest catalytic efficiency is ENLYFQ ▼S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1).

  • Removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins
  • Engineered to prevent autolysis and improve stability
  • Optimal activity and stability for up to 24 months
  • Active in a wide range of buffers; optimal activity between pH 6.0 and 9.0.
  • High substrate specificity with no non-specific proteolysis
  • TEV Protease contains a 7x His-Tag for easy removal from a reaction using NEBExpress® Ni Resin (NEB# S1428S), NEBExpress® Ni Spin Columns (NEB# S1427S/L), or NEBExpress® Ni-NTA Magnetic Beads (NEB# S1423S)

TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ ▼S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1). It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. TEV Protease has a 7xHis-tag for easy removal from a reaction using nickel affinity resins and has been engineered to improve thermal stability and decrease autolysis. 

Product Source
Cloned from Tobacco Etch Virus and expressed in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  TEV Protease P8112SVIAL -20 1 x 0.1 ml 10,000 units/ml
  TEV Protease Reaction Buffer B8035SVIAL -20 1 x 1 ml 10 X
Features
  • Removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins
  • Engineered to improve thermal stability and decrease autolysis
  • High substrate specificity with no non-specific proteolysis 
 
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Notes
  • The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ ▼S; however, the amino acid in the P1’ position (S in the recognition sequence) can also be G, A, M, C, or H (1).
  • If the fusion protein sample contains > 2 M urea, > 0.5 M Guanidine hydrochloride, > 50 mM imidazole, pH values below 6 or above 9, or cysteine protease inhibitors then it will be necessary to dialyze the fusion protein before TEV Protease cleavage.
  • TEV protease can be used at high concentrations without the occurrence of non-specific proteolysis.
  • Reactions may be scaled-up linearly to accommodate larger reaction volumes.
References
  • Kapust, R.B. et al. (2002). Biochem. and Biophysical Research Comm.. 294, 949-955.
Selection Charts
FAQs
Tech Tips
    • The optimal pH range is 6.0 – 8.0
    • Optimal activity achieved in ≤0.2M NaCl; however, the enzyme retains some activity in up to 2M NaCl
  • Cleavage at lower temperatures, such as 4ºC, requires overnight incubation

  • Inhibition occurs in the presence of ≥ 5 mM Zn2+, ≥ 1 mM Cu2+ and ≥ 10 mM Co2+

    • Compatible with 10mM MgSO4, MnCl2 and CaCl2 and up to 100mM EDTA
    • Compatible with the following protease inhibitors: aprotinin, benzamidine, leupeptin, pepstatin, PMSF
    • Can be used at high concentrations with no non-specific proteolysis occurring
    • Some substrates may require extended incubation (up to three days at either 4°C or 30°C)
    • If cleavage is not complete, add more TEV protease after 24 hours and continue incubation
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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