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Furin

Catalog # Concentration Size List Price Quantity Your Price
P8077L 2000 units/ml 250 units $982.00
$883.80
P8077S 2000 units/ml 50 units $247.00
$222.30
Catalog # Size List Price Your Price
P8077L 250 units $982.00
$883.80
P8077S 50 units $247.00
$222.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Furin is a recombinant, ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg˅. However, the enzyme prefers the site Arg-X-Lys/Arg-Arg˅. 

  • It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network
  • Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor
Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network (1,2). Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor. The minimal cleavage site is Arg-X-X-Arg'. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg'. An additional arginine at the P6 position appears to enhance cleavage (3). Furin is inhibited by EGTA, α1- Antitrypsin Portland (4) and polyarginine compounds (5).
Product Source
Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Furin P8077SVIAL -20 1 x 0.025 ml 2,000 units/ml
  Furin P8077LVIAL -20 1 x 0.125 ml 2,000 units/ml

Properties & Usage

Unit Definition

1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 µl.

Storage Buffer

10 mM MES
1 mM CaCl2
50% Glycerol
pH 7 @ 25°C

Molecular Weight

Theoretical: 52.7 kDa

Unit Assay Conditions

Two fold dilutions of Furin are incubated with 25 µg MBP-FN-paramyosin-ΔSal substrate in 20 mM HEPES, 0.1% Triton X-100, 1 mM CaCl2, 0.2mM β-mercaptoenthanol (pH 7.5 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 6 hours. Separation of reaction products are visualized by SDS-PAGE.

Companion Products
Notes
  • Both Furin and Onchocerca volvulus Blisterase will cleave peptide substrates with the sequence, Arg-X- (Lys/Arg)-Arg. However, the ability of either enzyme to cleave a particular protein substrate depends on its tertiary structure as well as on the amino acids immediately surrounding the cleavage site (6).
References
  • van den Ouweland, A.M.W. et al. (1990). Nucl. Acids Res. 18, 664.
  • Steiner, D.F. (1998). Curr. Opin. Chem. Biol.. 2,
  • Krysan, D.J. et al. (1999). J. Biol. Chem.. 274, 23229-23234.
  • Jean, F. et al. (1998). Proc. Natl. Acad. Sci. USA. 95, 7293-7298.
  • Cameron, A. et al. (2000). J. Biol. Chem.. 275, 36741-36749.
  • Poole, C.B. et al. (2003). J. Biol. Chem.. 278, 36183-36190.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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