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Sodium Orthovanadate (Vanadate)

Catalog # Concentration Size List Price Quantity Your Price
P0758L 100 mM 5 ml $206.00
$185.40
P0758S 100 mM 1 ml $55.00
$49.50
Catalog # Size List Price Your Price
P0758L 5 ml $206.00
$185.40
P0758S 1 ml $55.00
$49.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Sodium Orthovanadate (Vanadate) is a general competitive inhibitor for protein phosphotyrosyl phosphatases. The inhibition by Sodium Orthovanadate is reversible upon the addition of EDTA or by dilution.

  • Can be used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays
Sodium Orthovanadate (Vanadate, Na3VO4) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution. Vanadate has been activated for maximal inhibition of PTPs following the procedure described by J.A. Gordon (1).

The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays (1, 2). 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Sodium Orthovanadate (Vanadate) P0758SVIAL -20 1 x 1 ml 100 mM
  Sodium Orthovanadate (Vanadate) P0758SVIAL -20 5 x 1 ml 100 mM

Properties & Usage



Notes
  • Common buffer components such as EDTA and reducing agents may interact with Vanadate, affecting its potency (2).
  • Storage: Sterile purified water adjusted to pH 10.0 (1,2).
References
  • Gordon, J.A. (1991). Methods in Enzymology. 201, 477-482.
  • Huyer, G. et al. (1997). J. Biol. Chem.. 272, 843-851.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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