Protocol for Thermostable Endonuclease Q (NEB #M0701)

1. Reaction set-up:
   
   

   COMPONENTS	VOLUME
   dX, dI, dU-containing DNA substrate	1 pmol*
   NEBuffer™ r2.1 (10X)	2 µl
   Thermostable Endonuclease Q	1 µl
   Nuclease-free Water	Up to 20 µl

   
   
   
2. Incubate at 65-85°C for 1 hour (Thermostable Endonuclease Q will function at temperatures between 37-90°C, but functions optimally between 65-85°C.).

*Varies by substrate; please refer to www.neb.com for pmol requirements for different substrates.