Buffer Preparation Guidance for Monarch^® Spin DNA Gel Extraction Kit (NEB #T1120)

Add ethanol to the Monarch Buffer WZ prior to use (4 volumes of ≥ 95% ethanol per volume of Buffer WZ).

• For T1130S (50-prep) kit, add 20 ml of ethanol to Buffer WZ.
  
  
• For T1130L (250-prep) kit, add 104 ml of ethanol to Buffer WZ.

Always keep all buffer bottles tightly closed when not actively in use.