Protocol for XRN-1 NEB M0338
- Set-up the reaction as follows:
- Mix thoroughly.
- Incubate at 37°C for 60 minutes.
| COMPONENTS | 20 μl REACTION |
|---|---|
| Single stranded 5’phos-RNA | 1μg |
| NEBuffer 3 (10X) | 2 μl (1X) |
| Nuclease-free H2O (NEB #B1500) | up to 20 μl |
| XRN-1 (1,000 U/ml) | 2 μl |