Home Protocols Typical DNase I-XT Reaction Protocol (NEB #M0570)

Typical DNase I-XT Reaction Protocol (NEB #M0570)

  1. Set up the following reaction on ice:
    COMPONENTS 100 μl REACTION
     RNA ~ 10 μg RNA
     DNase I-XT Reaction Buffer (10X) 10 μl (1X)
     DNase I-XT  1 μl (2 units)
     Nuclease-free H2O  to 100 μl
  2. Incubate at 37°C for 15 minutes.
Note: When using RNA in downstream applications, column purification with NEB's Monarch RNA Cleanup Kits (NEB #T2030, #T2040, #T2050), or cleanup with phenol/chloroform extraction is recommended.