Protocol for T4 PDG (M0308)
1) Reaction set-up:
| DNA | 10 pmol |
| T4 PDG Reaction Buffer (10X) | 2-5 µl |
| T4 PDG (T4 Endonuclease V) | 1 µl (10 units) |
| Recombinant Albumin | 0.5 - 2.5 µl |
| H2O | Up to 20-50 µl |
2) Incubate at 37°C for 30 minutes.
Addition of SDS to a final concentration of 0.5%, or 1µl phenol, in 6X Loading dye to the sample before running the gel will improve electrophoresis by dissociating the protein from the DNA.