Home Protocols Typical Reaction Conditions for Endoproteinase LysC (P8109)

Typical Reaction Conditions for Endoproteinase LysC (P8109)

For difficult to digest proteins or general proteomics applications, substrate may be reduced, denatured and alkylated prior to LysC digest.
  1. Combine 10-100 ng/μl substrate protein, 10 mM DTT, 30 mM iodoacetic acid and 5-8 M urea.
  2. Incubate in the dark 30 minutes.
  3. Add LysC; 1:20–1:100 LysC : Substrate ratio by mass.
  4. Incubate reaction at 37°C for 2-18 hours.