Home Protocols First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)

First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)

Mix the following components and incubate at 42°C for 1 hour.

COMPONENT	VOLUME
Template RNA	up to 1 µg*
d(T)₂₃VN (50 µM) or Random Primer Mix (60 µM)	2 µl
5X ProtoScript II buffer	4 µl
0.1 M DTT	2 µl
10 mM dNTP Mix	1 µl
RNase Inhibitor (40 U/µl)	0.2 µl
Nuclease-free H₂O	To a total volume of 20 µl

* 1 ng-1 µg total RNA or 50 pg-100 ng poly(A)-RNA