Home Protocols Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)

Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)

Optimal incubation times and enzyme concentrations may need to be determined for a particular IgG. Typical reaction conditions are as follows:
  1. Combine 50 µg of native IgG, 2.5 µl of 10X GlycoBuffer 2 and H2O (if necessary) to make a 25 µl total reaction volume. 
  2. Add 1 µl of IdeZ Protease (IgG-specific). 
  3. Incubate at 37°C for 30 minutes*. 
*Extend incubation time to 2 hours for mouse IgG2a