Typical RT-LAMP Protocol
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Component Volume Final Concentration 10X Isothermal Amplification Buffer 2.5 µl 1X MgSO4 (100 mM) 1.5 µl 6 mM (8 mM total) dNTP Mix (10 mM) 3.5 µl 1.4 mM FIP/BIP Primers (25X) 1.6 µl 1.6 µM F3/B3 Primers (25X) 0.2 µl 0.2 µM LoopF/B Primers (25X) 0.4 µl 0.4 µM Bst 2.0® or Bst 2.0
WarmStart® DNA Polymerase1 µl 8 units WarmStart RTx Reverse Transcriptase 0.5 µl 7.5 units
Template X µl Total Reaction Volume 25 µl 25 µl - Mix by vortexing.
- Place directly at 65°C.