Typical cDNA Synthesis Protocol with WarmStart RTx (NEB #M0380)
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Component Final Conc./AMT. 10X Isothermal Amplification Buffer 1X dNTP Mix (10 mM) 0.5 mM Random Primer Mix 6 µM RNase Inhibitor, Murine
20 units WarmStart RTx Reverse Transcriptase 0.25 µl Total Reaction Volume 20 µl - Mix by vortexing.
- Incubate for 5 minutes at 25°C for annealing and 10 minutes at 55°C for synthesis.
- Heat Inactivate WarmStart RTx Reverse Transcriptase by incubation at 80°C for 10 minutes.