Standard PCR Protocol (E5315)
| CYCLE STEP | TEMPERATURE | TIME | CYCLES |
| Reverse Transcription | 48°C | 15–30 minutes | 1 |
| Initial Denaturation | 94°C | 1 minute | 1 |
| Denaturation Annealing Extension |
94°C 50–65°C 68°C |
15 seconds 30 seconds 1 minute per kb |
40 |
| Final Extension | 68°C | 5 minutes | 1 |
| Hold | 4°C | ∞ | 1 |