FAQ: How do I quantify my WGA products generated using the phi29-XT WGA Kit (NEB #E1604)?

WGA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. Alternatively, purified WGA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®.
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