Recovery of DNA from 50 bp to 25 kb can be achieved with the Monarch Spin Columns S1A, supplied in both the Monarch Spin DNA Gel Extraction Kit (NEB #T1120) and the Monarch Spin PCR & DNA Cleanup Kit (5 µg) (NEB #T1130). Efficiency decreases with longer DNA due to tighter binding to the matrix. Recovery with longer DNA can be increased with modified elution methods such as using heated elution buffer (50°C) and/or incubating at room temperature for 5 minutes after the addition of elution buffer. For detailed instructions, please read the instructions provided in the manual.