A reaction is defined as 1 µl of Authenticase in a 20 µl reaction containing 1X Authenticase Reaction Buffer, a mixture of 0.33 pmol of 60-mer heteroduplex of A/C, T/G and 2 bp indel (insertion and/or deletion) mismatches, respectively. After incubation at 42°C for 30 min, >90% of the substrate DNA was digested, as determined by 4% agarose E-gel. More details of the Authenticase protocol and usage can be found in the product manual.