FAQ: Does NEB provide plasmids for sgRNA cloning?

We do not distribute plasmids for sgRNA cloning, but we recommend that you visit Addgene if you wish to obtain sgRNA plasmids.

DNA templates containing a T7 promoter and encoding a sgRNA can be used with the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB #E2050).

Additionally, there are many available commercial sources of synthetic sgRNA.

Top