Yes, NEBExpress^® T4 Lysozyme is compatible with commonly used reducing reagents, including β-mercaptoethanol, DTT, TCEP, and THP. For downstream purification, unlike other vendors, NEBExpress^® Nickel Spin Columns (NEB #S1427) and NEBExpress^® Nickel Resin (NEB #S1428) are chemically resistant to these reducing reagents.