Thermostable OGG is active between 25-80°C, however optimal activity is observed between 65-75°C.
| Temperature (°C) | Units/ml |
|---|---|
| 16 | 1000 |
| 25 | 1000 |
| 37 | 2500 |
| 42 | 4500 |
| 50 | 5500 |
| 60 | 7000 |
| 65 | 8000 |
| 70 | 8000 |
| 75 | 8000 |
| 80 | 6000 |
Activity at different temperatures is based on the unit definition assay: The amount of enzyme required to cleave 10 pmol of a 60-mer fluorescently labeled oligonucleotide duplex containing a single 8-oxoguanine base paired with cytosine in a total reaction volume of 10 μl in 1 hour.