| Buffer | % Activity1 |
|---|---|
| NEBuffer r1.1 | 50% |
| NEBuffer r2.1 | 100% |
| NEBuffer r3.1 | 25% |
| rCutSmart™ Buffer | 75% |
| Q5® Reaction Buffer2 | 0% |
| ThermoPol Reaction Buffer2 | 25% |
| Standard Taq Reaction Buffer2 | 75% |
| Phusion HF Buffer2 | 100% |
1 % Activity is listed in comparison to the optimal buffer, NEBuffer r2.1.
2 Supplemented with 10 mM MgCl2