Quick CIP (NEB #M0525), rSAP (NEB #M0371) are active in all restriction enzyme NEBuffers r1.1, r2.1, r3.1 and rCutSmart™ Buffer (NEB #B6004) and can be added directly to digested DNA.. Antarctic Phosphatase (NEB #M0289) has a strict requirement for zinc and has optimal activity at pH 6.0.
Antarctic Phosphatase can be used in NEBuffers 1, 2, 3 or 4 as well as the unique NEBuffers for EcoRI and BamHI only when supplemented with 10X Antarctic Phosphatase Reaction Buffer to a final concentration of 1X.