Yes, Endoproteinase LysC can be used in acidic buffer conditions. Robust cleavage yielding the products has been observed in the following buffers:
- 20 mM sodium acetate pH 4.5
- 20 mM sodium acetate pH 5.5
- 20 mM sodium acetate pH 6.0
- 20 mM sodium acetate pH 6.5
Digests can also be run in Tris buffer between a pH range of 7.0 - 9.1.