Yes, this product is a good choice for colony PCR. Use of the Quick-Load Buffer permits direct loading onto an agarose gel. Please note that we recommend an initial 2-5 min denaturation step to lyse cells.
For convenience, use a master mix formulation such as OneTaq Quick-Load 2X Master Mix with Standard Buffer or the hot start equivalent OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer. An application note describes the protocol in detail.