NEB does not offer sgRNA synthesis services or plasmid for sgRNA cloning. For cloning we recommend visiting Addgene to obtain sgRNA plasmids.

For quickly transcribing sgRNAs without the need for cloning or PCR, we recommend the EnGen sgRNA Synthesis Kit, S. pyogenes (E3322S).

Alternatively, oligonucleotide templates encoding an sgRNA under a T7 promoter (PCR products or plasmids) can be used to transcribe sgRNA using an in vitro transcription kit such as NEB's HiScribe T7 Quick High Yield RNA Synthesis Kit (E2050).