Prepare a 20 µL mix containing: 2 µL Isothermal Amplification Buffer (1X) 0.5 mM dNTP 6 µM Random Primer Mix 20 U Murine RNase Inhibitor 3.75 U WarmStart® RTx Mix, incubate 5 min at 25°C for annealing and 10 min at 55°C for synthesis. Inactivate RTx with 10 min at 80°C.