Yes. T4 RNA Ligase 2, truncated KQ combines 2 active site mutations - R55K and K227Q, that improve the performance of this enzyme as compared to T4 RNA Ligase 2, truncated and T4 RNA Ligase 2, truncated K227Q. Ligation with T4 RNA Ligase 2, truncated KQ reduces levels of side ligation products to levels comparable to T4 RNA Ligase 2, truncated K227Q, while accumulating desired ligation products at rates similar to T4 RNA Ligase 2, truncated.