Bst 2.0 WarmStart® DNA Polymerase provides all the improved properties of using Bst 2.0 DNA Polymerase, with the additional benefit of room temperature reaction setup capability. DNA polymerases display undesired, non-templated activity at temperatures below set amplification temperatures and this activity can significantly affect performance and reproducibility. The aptamer in Bst 2.0 WarmStart® inhibits DNA polymerase activity below 45 °C and reactions can therefore be set up at room temperature.